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    An update on DNA analysis of faecal samples to assess diet of CEMP indicator species and steps to inform the review of Standard Methods

    Request Meeting Document
    Document Number:
    WG-EMM-2024/32
    Author(s):
    McInnes, J.C., B.E. Deagle, M.J. Dunn, L. Emmerson, A.J. MacDonald, G. Mergard, A. Polanowski, L. Suter and C.M. Waluda
    Submitted By:
    Dr Louise Emmerson (Australia)
    Approved By:
    Dr Philippe Ziegler (Australia)
    Abstract
    • Key life history parameters of selected indicator species are monitored as part of the CCAMLR Ecosystem Monitoring Program (CEMP). The diets of penguins are currently monitored using stomach lavage techniques to identify prey composition and mass. 
    • DNA metabarcoding of prey DNA in faeces (DNA dietary analysis) provides a complementary, non- invasive technique that yields high taxonomic resolution. Using a combination of genetic markers it is possible to determine the relative proportion of broad taxonomic groups (e.g. fish, crustaceans, molluscs etc.) in the diet, as well as species level resolution of prey. 
    • This paper provides an update on DNA dietary methods and an overview of current studies using these methods on seabirds and seals in the Southern Ocean. These methods are applicable to all CEMP indicator species and thus relevant for the CEMP review of Standard Methods. 
    • We outline the steps being taken to provide robust and repeatable standardised methodologies for Southern Ocean dietary studies and highlight relevant case studies to inform the review.